Pylsgrna-osu6a

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August undefined, 2024

http://www.biofeng.com/adjy/zhili/pYLsgRNA-OsU6a/LacZ.html Tīmeklis浩格生物科技有限公司是专业的p35s-spynehh-zw-048厂家,为您提供价格优惠最佳解决方案,我们有p35s-spynehh-zw-048各种型号、资料、使用说明pdf文档,采购p35s-spynehh-zw-048尽在浩格生物科技有限公司

CASE toolkit for easy and efficient multiplex transgene-free gene ...

Tīmeklis2024. gada 29. janv. · Overlapping PCR was performed to produce the sequence of sgRNA–OsU6a promoter. The PCR product with attB adaptors was then introduced into pDONR207 with a BP reaction, producing the donor vector named PJG090, which is used as the template for PCR amplification of two spacers. ... The first round PCR … TīmeklisPlant multiplex genome editing vector pYLsgRNA-OsU6a/LacZ Taxonomy ID: 1648247 (for references in articles please use NCBI:txid1648247) current name. Plant multiplex genome editing vector pYLsgRNA-OsU6a/LacZ. NCBI … reasors pharmacy 41st and yale tulsa

pEBB-3XMyc-TRAF2(Plasmid #44104)_海吉浩格生物,质粒 …

Tīmeklis2016. gada 19. febr. · Vectors (pYLsgRNA-OsU3, pYLsgRNA-OsU6a, pYLsgRNA-OsU6b, and pYLsgRNA-OsU6c for rice and pYLsgRNA-AtU3b, pYLsgRNA-AtU3d, … http://www.hedgehogbio.net/Products/D10711461.html http://www.biofeng.com/adjy/zhili/ university of maryland materials science

Pylsgrna-osu6a

http://www.labbase.net/Product/ProductItems-27-167-2187-79430.html Tīmeklis2024. gada 8. dec. · 取10μm靶位点前后引物各5μl混合后99℃退火5min并自然冷却获得含敲除靶位点的双链序列，然后利用bas1(neb公司)边酶切边连接(37℃)，将靶位点序列连至中间载体pylsgrna-osu6a上，随后利用通用扩增引物ctccgttttacctgtggaatcg(uf)和cggaggaaaattccatccac(gr-r)分别与靶位点后引物和前 ...

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TīmeklispYLsgRNA-OsU6a载体价格质粒购买价格是1200元，pYLsgRNA-OsU6a载体质粒图谱(Vector map)，载体序列(Sequence)见下文，载体质粒抗性为氨苄霉素。pYLsgRNA … TīmeklisThe first round PCR was set up with pYLsgRNA-OsU6a (Ma et al., 2016) as the template . Figure 1: Construction of the donor vectors as the templates for multiplex …

TīmeklispYLsgRNA-OsU6a Sequences (3) Addgene Sequences: Partial (2) Depositing Scientist Sequences: Full (1) Full Sequences from Depositor (1) Sequence provided by … TīmeklisPlasmid pYLsgRNA-OsU6a from Dr. Yao-Guang Liu's lab is published in Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 10.1016/j.molp.2015.04.007. This …

Tīmeklis2024. gada 4. jūn. · pYLsgRNA intermediates (PYLsgRNA-OsU6a/Lac, PYLsgRNA-OsU6b, PYLsgRNA-OsU6c, and PYLsgRNA-OsU3). 2. pYLCRISPR/Cas9P ubi-H binary vector. 3. Restriction enzymes. 4. T 4 DNA ligase. 5. Gibson Assembly Cloning Kit. 6. 2 × Taq Master Mix. 7. Chimeric primers with target sequence and universal … http://www.shhebio.com/page53?article_id=59816

Tīmeklis2024. gada 21. febr. · pMSCV-CasRx-gRNA-puro 慢病毒质粒真核表达质粒原核表达质粒报告基因质粒自噬质粒双分子荧光抗体表达质粒基因辑质粒，酵母相关质粒植 …

Tīmeklis本发明公开了通过抑制SD1基因的表达降低四倍体野生稻株高的方法。本发明的具体实施例证明通过编辑绿色革命基因SD1，可实现株高的快速驯化，能使四倍体野生 … reasors pharmacy broken arrow 71st lynn laneTīmeklisThe sgRNA scaffold was amplified from pYLsgRNA-OsU6a vector (Ma et al., 2015) and all MaPDS target sites (Wu et al., 2024) used in this study were introduced into … reasors pharmacy claremore reasors pharmacy jenks okTīmeklis2024. gada 7. janv. · Ma X, Liu Y. CRISPR/Cas9-Based Multiplex Genome Editing in Monocot and Dicot Plants. Curr Protoc Mol Biol. 2016;115:31.6.1-31.6.21 pubmed … reasors pharmacy langley okTīmeklis2024. gada 2. jūl. · The arrangement of sgRNA cassettes driven by U6 promoter-was as follows; LacZ-OsU6a-T1-OsU6b-T2 with corresponding sizes of 629 bp and 515 bp for T 1 and T 2 respectively (Supplementary file 1, ... pYLsgRNA-OsU6a (3289 bp); (b) cloning strategy of PYLsgRNA-OsU6a showing sticky ends created by cutting with … university of maryland masters in economicsTīmeklis2024. gada 10. jūn. · (c) PCR GXL of gRNA expression cassettes; pYLsgRNA-OsU6a and pYLsgRNA-OsU6b ampli ed using primers gR-R/gRNA target adapter forward … reasors pharmacy tahlequahTīmeklis2015. gada 14. apr. · (B) The two Bsa I cutting sequences are given in twelve sgRNA vectors (in a linear form), including other four vectors (pYLsgRNA-OsU3/LacZ, … university of maryland math